Isolation, characterization and molecular identification of bacteria from tannery effluent using 16S rRNA sequencing

The present study deals with isolation, identification and analysis of bacteria from tannery effluent through 16S rRNA based molecular technique. Bacterial strain was isolated and characterized using various biochemical tests and confirmed through molecular approach. Bacterial 16S rRNA gene was amplified using suitable primers. The amplified 16S rRNA gene sequence was compared with the sequence in NCBI sequence database. The bacterial strain was identified as Bacillus thuringiensis strain IAM 12077 (NCBI Gene Bank Accession No: NR_043403.1) Phylogenetic and molecular evolutionary analyses were conducted using 16S rRNA sequencing. Bacillus thuringiensis sp. is a gram positive, oval shaped bacteria which forms white raised irregular colony. The sequence when submitted to NCBI gene bank database using BLAST showed 99 – 100% maximum identity and E-value equal to 0 for all closely related taxa.